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p415-GalL-Cas9-CYC1t
(Plasmid #43804)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 43804 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p415
  • Backbone size w/o insert (bp) 6670
  • Total vector size (bp) 10810
  • Vector type
    Yeast Expression, CRISPR
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Human Optimized S. pyogenes Cas9
  • Species
    H. sapiens (human), S. cerevisiae (budding yeast); S.pyogenes
  • Insert Size (bp)
    4140
  • Promoter GalL

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Plasmid 41815 from Prashant Mali
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For more information on Church Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/church/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p415-GalL-Cas9-CYC1t was a gift from George Church (Addgene plasmid # 43804 ; http://n2t.net/addgene:43804 ; RRID:Addgene_43804)

  • For your References section:

    Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems. Dicarlo JE, Norville JE, Mali P, Rios X, Aach J, Church GM.. Nucleic Acids Res 10.1093/nar/gkt135 PubMed 23460208
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