p426-SNR52p-gRNA.CAN1.Y-SUP4t
(Plasmid #43803)

• Purpose: Encodes a gRNA that targets Can1.Y in yeast.
• Depositing Lab: George Church
• Publication: Dicarlo et al Nucleic Acids Res

Backbone

• Vector backbone: p426
• Backbone size w/o insert (bp): 5886
• Total vector size (bp): 6274
• Vector type: Yeast Expression, CRISPR
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CAN1.y gRNA
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 488
• Entrez Gene: CAN1 (a.k.a. YEL063C)
• Promoter: SNR52

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T3 promoter
• 3′ sequencing primer: T7 promoter

Resource Information

• Supplemental Documents:
  • Genbank File
• Articles Citing this Plasmid:
  • 55 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For more information on Church Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/church/

gRNA target sequence GATACGTTCTCTATGGAGGA