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pFRT/FLAG/HA-DEST LIN28A
(Plasmid #43797)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 43797 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFRT/FLAG/HA-DEST
  • Backbone size w/o insert (bp) 5372
  • Total vector size (bp) 6002
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LIN28A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    630
  • GenBank ID
    NP_078950.1
  • Entrez Gene
    LIN28A (a.k.a. CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A)
  • Tag / Fusion Protein
    • FLAG-HA (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFRT/FLAG/HA-DEST LIN28A was a gift from Thomas Tuschl (Addgene plasmid # 43797 ; http://n2t.net/addgene:43797 ; RRID:Addgene_43797)

  • For your References section:

    Identification of mRNAs bound and regulated by human LIN28 proteins and molecular requirements for RNA recognition. Hafner M, Max KE, Bandaru P, Morozov P, Gerstberger S, Brown M, Molina H, Tuschl T. RNA. 2013 May;19(5):613-26. doi: 10.1261/rna.036491.112. Epub 2013 Mar 12. 10.1261/rna.036491.112 PubMed 23481595
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