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Addgene

pDawn Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid with the following enzyme(s): BamHI and XhoI.
We expected the following band sizes for this digest: 7.2kb. We see the expected pattern.

Addgene Diagnostic Test

This gel depicts the NEB 1kb ladder and a digestion of the plasmid 43796 with the following enzymes: EcoRI-HF
Our results indicate the presence of a second EcoRI site, as this digest gives 2 bands of ~5.2kb and 2kb.