Skip to main content
Addgene

CLPIT Cry2PHR-mCh-RhoA
(Plasmid #42959)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42959 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    CLPIT
  • Backbone size w/o insert (bp) 7821
  • Total vector size (bp) 10656
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cry2PHR-mCherry-RhoA
  • Species
    H. sapiens (human), A. thaliana (mustard weed)
  • Insert Size (bp)
    2835
  • Entrez Gene
    RHOA (a.k.a. ARH12, ARHA, EDFAOB, RHO12, RHOH12)
  • Promoter Tet-Off promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SfiI (not destroyed)
  • 3′ cloning site PmeI (not destroyed)
  • 5′ sequencing primer AGACGCCATCCAACGCTGTTT
  • 3′ sequencing primer AGAGCCTGGACCACTGATATCCTGTCT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CLPIT Cry2PHR-mCh-RhoA was a gift from David Schaffer (Addgene plasmid # 42959 ; http://n2t.net/addgene:42959 ; RRID:Addgene_42959)

  • For your References section:

    Optogenetic protein clustering and signaling activation in mammalian cells. Bugaj LJ, Choksi AT, Mesuda CK, Kane RS, Schaffer DV. Nat Methods. 2013 Feb 3. doi: 10.1038/nmeth.2360. 10.1038/nmeth.2360 PubMed 23377377
Related items:
Commonly requested with: