pCry2PHR-mCh-RhoA
(Plasmid #42958)

• Purpose: Expresses optogenetically controlled RhoA
• Depositing Lab: David Schaffer
• Publication: Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nmeth.2360.

Backbone

• Vector backbone: pmCherry-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3991
• Total vector size (bp): 6826
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cry2PHR-mCherry-RhoA
• Species: H. sapiens (human), A. thaliana (mustard weed)
• Insert Size (bp): 2835
• Entrez Gene: RHOA (a.k.a. ARH12, ARHA, EDFAOB, RHO12, RHOH12)
• Promoter: CMV

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CMV-F

Resource Information

• Supplemental Documents:
  • Notes from depositor--experimental considerations
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry