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Addgene

SOX2 TALE Repressor (KRAB 1-75)
(Plasmid #42945)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42945 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLenti
  • Total vector size (bp) 12789
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SOX2 TALE Repressor (KRAB 1-75)
  • Alt name
    SOX2 TALE KRAB
  • Alt name
    TALE Repressor
  • Alt name
    KRAB 1-75 truncation
  • Promoter EF-1a
  • Tags / Fusion Proteins
    • 2A-GFP (C terminal on insert)
    • 2A-GFP (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is a new collection of constructs described in Cong et al. Nature Communications.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SOX2 TALE Repressor (KRAB 1-75) was a gift from Feng Zhang (Addgene plasmid # 42945 ; http://n2t.net/addgene:42945 ; RRID:Addgene_42945)
  • For your References section:

    Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains. Cong L, Zhou R, Kuo YC, Cunniff M, Zhang F. Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962. 10.1038/ncomms1962 PubMed 22828628