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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 42945 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLenti
- Total vector size (bp) 12789
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSOX2 TALE Repressor (KRAB 1-75)
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Alt nameSOX2 TALE KRAB
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Alt nameTALE Repressor
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Alt nameKRAB 1-75 truncation
- Promoter EF-1a
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Tags
/ Fusion Proteins
- 2A-GFP (C terminal on insert)
- 2A-GFP (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer EF-1a-Forward
- 3′ sequencing primer WPRE-Reverse (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This is a new collection of constructs described in Cong et al. Nature Communications.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SOX2 TALE Repressor (KRAB 1-75) was a gift from Feng Zhang (Addgene plasmid # 42945 ; http://n2t.net/addgene:42945 ; RRID:Addgene_42945) -
For your References section:
Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains. Cong L, Zhou R, Kuo YC, Cunniff M, Zhang F. Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962. 10.1038/ncomms1962 PubMed 22828628