pPET-PKR/PPase
(Plasmid #42934)

• Depositing Lab: James Cole
• Publication: Lemaire et al J Mol Biol. 2005 Jan 7;345(1):81-90.

Backbone

• Vector backbone: pet11a
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: protein kinase R
• Alt name: PKR
• Alt name: EIF2AK2
• Species: H. sapiens (human)
• Insert Size (bp): 1653
• GenBank ID: NM_002759.3
• Entrez Gene: EIF2AK2 (a.k.a. DYT33, EIF2AK1, LEUDEN, PKR, PPP1R83, PRKR)
• Promoter: T7

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7 promotor
• 3′ sequencing primer: T7 terminator

Gene/Insert 2

• Gene/Insert name: phage lambda phosphatase
• Species: phage lambda
• Insert Size (bp): 663

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BlpI (not destroyed)
• 5′ sequencing primer: T7 promotor
• 3′ sequencing primer: T7 terminator

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry