pPV476
(Plasmid
#42929)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 42929 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR P2R-P3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4773
- Total vector size (bp) 4113
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Vector typeMulti-site Gateway donor plasmid
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameattR2::egfp (870 bp)::taa::Ss-era-1 terminator (598 bp)::attL3
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Alt nameEGFP
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Alt nameSs-era-1 terminator
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Alt nameera-1
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SpeciesStrongyloides stercoralis
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Insert Size (bp)1500
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13_pUC_fwd
- 3′ sequencing primer M13_rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byegfp (870 bp) from pJA257 (Addgene plasmid #21510, Julie Ahringer lab), Ss-era-1 terminator (598 bp) from pAJ08 (Addgene plasmid #14912, James Lok lab)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPV476 was a gift from James Lok (Addgene plasmid # 42929 ; http://n2t.net/addgene:42929 ; RRID:Addgene_42929)