PCx35-pcDNA-S110A, S276A, S298ter
(Plasmid #42927)

• Depositing Lab: John O'Brien
• Publication: Ouyang et al Brain Res Mol Brain Res. 2005 Apr 27;135(1-2):1-11.

Backbone

• Vector backbone: pcDNA3.1 Zeo
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4990
• Total vector size (bp): 6107
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Connexin 35
• Alt name: Cx35
• Alt name: Cx36
• Alt name: gjd2
• Species: Perch (Morone americana)
• Insert Size (bp): 1117
• Mutation: Ser110 to Ala, Ser276 to Ala, and Ser298 to stop codon
• GenBank ID: AF059183
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PstI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: BGH reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains non-phosphorylatable point mutations at Ser110 in the cytoplasmic loop and Ser276 in the C-terminal domain, and stop codon at Ser298 truncating the last 7 amino acids. This construct has essentially no regulation by phosphorylation.

Derived from clone from: O'Brien, J., Nguyen, H.B., and Mills, S.L. (2004). Cone photoreceptors in bass retina use two connexins to mediate electrical coupling. J Neurosci 24, 5632-5642.

Original cDNA clone from: O'Brien, J., Bruzzone, R., White, T.W., Al-Ubaidi, M.R., and Ripps, H. (1998). Cloning and expression of two related connexins from the perch retina define a distinct subgroup of the connexin family. J Neurosci 18, 7625-7637.