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PCx35-pcDNA-S276A
(Plasmid #42924)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42924 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1 Zeo
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4990
  • Total vector size (bp) 6107
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Connexin 35
  • Alt name
    Cx35
  • Alt name
    Cx36
  • Alt name
    gjd2
  • Species
    Perch (Morone americana)
  • Insert Size (bp)
    1117
  • Mutation
    Ser276 to Ala
  • GenBank ID
    AF059183
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer BGH reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains a non-phosphorylatable point mutation in Ser276 in the C-terminal domain. Phosphorylation of this site regulates coupling.

Derived from clone from: O'Brien, J., Nguyen, H.B., and Mills, S.L. (2004). Cone photoreceptors in bass retina use two connexins to mediate electrical coupling. J Neurosci 24, 5632-5642.

Original cDNA clone from: O'Brien, J., Bruzzone, R., White, T.W., Al-Ubaidi, M.R., and Ripps, H. (1998). Cloning and expression of two related connexins from the perch retina define a distinct subgroup of the connexin family. J Neurosci 18, 7625-7637.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PCx35-pcDNA-S276A was a gift from John O'Brien (Addgene plasmid # 42924 ; http://n2t.net/addgene:42924 ; RRID:Addgene_42924)

  • For your References section:

    Protein kinase A mediates regulation of gap junctions containing connexin35 through a complex pathway. Ouyang X, Winbow VM, Patel LS, Burr GS, Mitchell CK, O'Brien J. Brain Res Mol Brain Res. 2005 Apr 27;135(1-2):1-11. 10.1016/j.molbrainres.2004.10.045 PubMed 15857663
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