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PCx34.7IL-pET42
(Plasmid #42915)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42915 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET42c
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5930
  • Total vector size (bp) 6168
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Requires a DE3 strain for expression of protein.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Connexin 34.7 cytoplasmic loop
  • Alt name
    Cx34.7
  • Species
    Perch (Morone americana)
  • Insert Size (bp)
    243
  • Mutation
    cytoplasmic loop is aa 102-179
  • GenBank ID
    AF059184
  • Promoter T7
  • Tags / Fusion Proteins
    • GST (N terminal on backbone)
    • 6x His (N terminal on backbone)
    • S-tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (destroyed during cloning)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer S-tag
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Original cDNA clone is from O'Brien, J., Bruzzone, R., White, T.W., Al-Ubaidi, M.R., and Ripps, H. (1998). Cloning and expression of two related connexins from the perch retina define a distinct subgroup of the connexin family. J Neurosci 18, 7625-7637.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PCx34.7IL-pET42 was a gift from John O'Brien (Addgene plasmid # 42915 ; http://n2t.net/addgene:42915 ; RRID:Addgene_42915)

  • For your References section:

    Calcium-dependent binding of calmodulin to neuronal gap junction proteins. Burr GS, Mitchell CK, Keflemariam YJ, Heidelberger R, O'Brien J. Biochem Biophys Res Commun. 2005 Oct 7;335(4):1191-8. 10.1016/j.bbrc.2005.08.007 PubMed 16112650
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