PCx35CT-S276A, S298ter
(Plasmid #42914)

• Depositing Lab: John O'Brien
• Publication: Ouyang et al Brain Res Mol Brain Res. 2005 Apr 27;135(1-2):1-11.

Backbone

• Vector backbone: pET42c
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5930
• Total vector size (bp): 6067
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Requires a DE3 strain for expression of protein.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Connexin 35 C-terminal domain
• Alt name: Cx35
• Alt name: Cx36
• Alt name: gjd2
• Species: Perch (Morone americana)
• Insert Size (bp): 182
• Mutation: Ser276 to Ala and Ser298 stop codon
• GenBank ID: AF059183
• Promoter: T7
• Tags / Fusion Proteins:
  • GST (N terminal on backbone)
  • 6x His (N terminal on backbone)
  • S-tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: S-tag
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Non-phosphorylatable point mutation at major PKA phosphorylation site on C-terminus plus stop codon at CamKII phosphorylation site near tip of the CT. Truncates last 7 amino acids.