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Addgene

PCx35CT-S276A, S298ter
(Plasmid #42914)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42914 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET42c
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5930
  • Total vector size (bp) 6067
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Requires a DE3 strain for expression of protein.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Connexin 35 C-terminal domain
  • Alt name
    Cx35
  • Alt name
    Cx36
  • Alt name
    gjd2
  • Species
    Perch (Morone americana)
  • Insert Size (bp)
    182
  • Mutation
    Ser276 to Ala and Ser298 stop codon
  • GenBank ID
    AF059183
  • Promoter T7
  • Tags / Fusion Proteins
    • GST (N terminal on backbone)
    • 6x His (N terminal on backbone)
    • S-tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer S-tag
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Non-phosphorylatable point mutation at major PKA phosphorylation site on C-terminus plus stop codon at CamKII phosphorylation site near tip of the CT. Truncates last 7 amino acids.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PCx35CT-S276A, S298ter was a gift from John O'Brien (Addgene plasmid # 42914 ; http://n2t.net/addgene:42914 ; RRID:Addgene_42914)

  • For your References section:

    Protein kinase A mediates regulation of gap junctions containing connexin35 through a complex pathway. Ouyang X, Winbow VM, Patel LS, Burr GS, Mitchell CK, O'Brien J. Brain Res Mol Brain Res. 2005 Apr 27;135(1-2):1-11. 10.1016/j.molbrainres.2004.10.045 PubMed 15857663
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