PCx35CT-S298ter
(Plasmid
#42913)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 42913 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET42c
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5930
- Total vector size (bp) 6067
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsRequires a DE3 strain for expression of protein.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameConnexin 35 C-terminal domain
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Alt nameCx35
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Alt nameCx36
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Alt namegjd2
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SpeciesPerch (Morone americana)
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Insert Size (bp)182
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MutationSer298 stop codon
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GenBank IDAF059183
- Promoter T7
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Tags
/ Fusion Proteins
- GST (N terminal on backbone)
- 6x His (N terminal on backbone)
- S-tag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer S-tag
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Stop codon at CamKII phosphorylation site near tip of the C-terminus. Truncates last 7 amino acids.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PCx35CT-S298ter was a gift from John O'Brien (Addgene plasmid # 42913 ; http://n2t.net/addgene:42913 ; RRID:Addgene_42913) -
For your References section:
Protein kinase A mediates regulation of gap junctions containing connexin35 through a complex pathway. Ouyang X, Winbow VM, Patel LS, Burr GS, Mitchell CK, O'Brien J. Brain Res Mol Brain Res. 2005 Apr 27;135(1-2):1-11. 10.1016/j.molbrainres.2004.10.045 PubMed 15857663