pcDNAI Neo human PECAM FL
(Plasmid
#42902)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 42902 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNAI Neo
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 7000
- Total vector size (bp) 9500
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePECAM
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Alt nameplatelet/endothelial cell adhesion molecule 1
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Alt namePECAM-1
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Alt nameCD31/EndoCAM
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SpeciesH. sapiens (human)
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Insert Size (bp)2557
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GenBank ID
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Entrez GenePECAM1 (a.k.a. CD31, CD31/EndoCAM, GPIIA', PECA1, PECAM-1, endoCAM)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (unknown if destroyed)
- 3′ cloning site HindIII (unknown if destroyed)
- 5′ sequencing primer CMV-F; T7
- 3′ sequencing primer Sp6 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNAI Neo human PECAM FL was a gift from Steven Albelda (Addgene plasmid # 42902 ; http://n2t.net/addgene:42902 ; RRID:Addgene_42902)