pcDNAI Neo mouse PECAM FL
(Plasmid #42901)

• Depositing Lab: Steven Albelda
• Publication: Albelda lab PECAM plasmids (unpublished)

Backbone

• Vector backbone: pcDNAI Neo
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 7000
• Total vector size (bp): 9500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PECAM
• Alt name: platelet/endothelial cell adhesion molecule 1
• Alt name: PECAM-1
• Alt name: Cd31
• Species: M. musculus (mouse)
• Insert Size (bp): 2534
• GenBank ID: L06039.1 AAA16230.1
• Entrez Gene: Pecam1 (a.k.a. Cd31, PECAM-1, Pecam)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (unknown if destroyed)
• 3′ cloning site: NsiI (unknown if destroyed)
• 5′ sequencing primer: CMV-F; T7
• 3′ sequencing primer: Sp6

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Compared to GenBank accession number L06039.1, the PECAM-1 sequence in this plasmid contains 2 nucleotide mismatches in the 5' untranslated region (UTR).