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Addgene

pCT-L7.5.1
(Plasmid #42900)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42900 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCT
  • Backbone size w/o insert (bp) 6200
  • Total vector size (bp) 6509
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    L7.5.1
  • Alt name
    FN3 Lysozyme Binder
  • Insert Size (bp)
    309
  • Promoter Gal1-10
  • Tags / Fusion Proteins
    • HA (N terminal on insert)
    • c-myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer pCT-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCT-L7.5.1 was a gift from Dane Wittrup (Addgene plasmid # 42900 ; http://n2t.net/addgene:42900 ; RRID:Addgene_42900)

  • For your References section:

    Picomolar affinity fibronectin domains engineered utilizing loop length diversity, recursive mutagenesis, and loop shuffling. Hackel BJ, Kapila A, Wittrup KD. J Mol Biol. 2008 Sep 19;381(5):1238-52. doi: 10.1016/j.jmb.2008.06.051. Epub 2008 Jun 24. 10.1016/j.jmb.2008.06.051 PubMed 18602401
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