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pcDNA 3.1/V5-His TOPO TA mouse Mns1
(Plasmid #42899)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42899 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA 3.1/V5-His TOPO TA
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Mns1
  • Alt name
    meiosis-specific nuclear structural protein 1
  • Alt name
    AW546487
  • Species
    M. musculus (mouse)
  • GenBank ID
    NM_008613.3 NP_032639.1
  • Entrez Gene
    Mns1 (a.k.a. AW546487)
  • Promoter CMV
  • Tags / Fusion Proteins
    • V5 (C terminal on backbone)
    • 6xHis (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The cDNA encoding full-length mouse Mns1, complete CDS with Kozak sequence, was cloned into pcDNA 3.1/V5-His TOPO TA (Invitrogen) for V5 tagging.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA 3.1/V5-His TOPO TA mouse Mns1 was a gift from Jeremy Wang (Addgene plasmid # 42899 ; http://n2t.net/addgene:42899 ; RRID:Addgene_42899)

  • For your References section:

    MNS1 is essential for spermiogenesis and motile ciliary functions in mice. Zhou J, Yang F, Leu NA, Wang PJ. PLoS Genet. 2012;8(3):e1002516. doi: 10.1371/journal.pgen.1002516. Epub 2012 Mar 1. 10.1371/journal.pgen.1002516 PubMed 22396656
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