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Addgene

pNC1011
(Plasmid #42898)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42898 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFA6aHis3MX6
  • Backbone manufacturer
    Longtine et. al. 1998 Yeast 14:953-961
  • Backbone size w/o insert (bp) 3820
  • Total vector size (bp) 6212
  • Vector type
    Yeast Integrating
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    3 tandem copies of GFP*
  • Species
    Aequorea victoria
  • Insert Size (bp)
    2163
  • Mutation
    changed Phenylalanine 64 to Leucine, Serine 65 to Threonine, Valine 163 to Alanine; Silent mutation at the Histidine 77 codon (CAT to CAC) that destroys an NdeI site
  • Promoter None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer CGG ATC CCC GGG TTA ATT AA
  • 3′ sequencing primer ACC TGA GAA AGC AAC C
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNC1011 was a gift from Beverly Errede (Addgene plasmid # 42898 ; http://n2t.net/addgene:42898 ; RRID:Addgene_42898)

  • For your References section:

    An improved short-lived fluorescent protein transcriptional reporter for Saccharomyces cerevisiae. Houser JR, Ford E, Chatterjea SM, Maleri S, Elston TC, Errede B. Yeast. 2012 Dec;29(12):519-30. doi: 10.1002/yea.2932. Epub 2012 Nov 21. 10.1002/yea.2932 PubMed 23172645
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