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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 42897 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNKX3.1
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Alt nameNK3 homeobox 1
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SpeciesH. sapiens (human)
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Entrez GeneNKX3-1 (a.k.a. BAPX2, NKX3, NKX3.1, NKX3A)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This is a full length NKX3.1 cDNA construct.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-NKX3.1 was a gift from Edward Gelmann (Addgene plasmid # 42897 ; http://n2t.net/addgene:42897 ; RRID:Addgene_42897) -
For your References section:
DNA-binding sequence of the human prostate-specific homeodomain protein NKX3.1. Steadman DJ, Giuffrida D, Gelmann EP. Nucleic Acids Res. 2000 Jun 15;28(12):2389-95. 10.1093/nar/28.12.2389 PubMed 10871372