His-NKX3.1
(Plasmid
#42896)
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 42896 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepET-30a
-
Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5400
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameNKX3.1
-
Alt nameNK3 homeobox 1
-
SpeciesH. sapiens (human)
-
Entrez GeneNKX3-1 (a.k.a. BAPX2, NKX3, NKX3.1, NKX3A)
-
Tag
/ Fusion Protein
- 6xHis (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This is a full length NKX3.1 cDNA construct. Protein expressed in E.coli BL21(DE3) competent cells
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
His-NKX3.1 was a gift from Edward Gelmann (Addgene plasmid # 42896 ; http://n2t.net/addgene:42896 ; RRID:Addgene_42896) -
For your References section:
Interactions of the acidic domain and SRF interacting motifs with the NKX3.1 homeodomain. Ju JH, Maeng JS, Lee DY, Piszczek G, Gelmann EP, Gruschus JM. Biochemistry. 2009 Nov 10;48(44):10601-7. doi: 10.1021/bi9013374. 10.1021/bi9013374 PubMed 19780584