3xFLAG-SENP6
(Plasmid
#42887)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 42887 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonep3xFLAG-CMV-10
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Backbone manufacturerSigma
- Backbone size w/o insert (bp) 6400
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSENP6
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Alt nameSUMO1/sentrin specific peptidase 6
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SpeciesH. sapiens (human)
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Insert Size (bp)3340
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Entrez GeneSENP6 (a.k.a. SSP1, SUSP1)
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Tag
/ Fusion Protein
- 3xFLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
3xFLAG-SENP6 was a gift from Edward Yeh (Addgene plasmid # 42887 ; http://n2t.net/addgene:42887 ; RRID:Addgene_42887) -
For your References section:
Regulation of DNA repair through deSUMOylation and SUMOylation of replication protein A complex. Dou H, Huang C, Singh M, Carpenter PB, Yeh ET. Mol Cell. 2010 Aug 13. 39(3):333-45. 10.1016/j.molcel.2010.07.021 PubMed 20705237