3xFLAG-SENP7
(Plasmid #42886)

• Depositing Lab: Edward Yeh
• Publication: Bawa-Khalfe et al Proc Natl Acad Sci U S A. 2012 Oct 23;109(43):17466-71. doi: 10.1073/pnas.1209378109. Epub 2012 Oct 8.

Backbone

• Vector backbone: p3xFLAG-CMV-10
• Backbone manufacturer: Sigma
• Backbone size w/o insert (bp): 6400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SENP7
• Alt name: SUMO1/sentrin specific peptidase 7
• Species: H. sapiens (human)
• Mutation: T102A, R969C, L996S
• Entrez Gene: SENP7
• Tag / Fusion Protein:
  • 3xFLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that Addgene's quality control sequence detects a T102A mutation in the insert of this plasmid. This mutation is not known to affect function.