pCas9 Citations (80)

Originally described in: RNA-guided editing of bacterial genomes using CRISPR-Cas systems.
Jiang W, Bikard D, Cox D, Zhang F, Marraffini LA Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2508. PubMed Journal

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Articles Citing pCas9

Articles
Coupling the CRISPR/Cas9 System with Lambda Red Recombineering Enables Simplified Chromosomal Gene Replacement in Escherichia coli. Pyne ME, Moo-Young M, Chung DA, Chou CP. Appl Environ Microbiol. 2015 Aug 1;81(15):5103-14. doi: 10.1128/AEM.01248-15. Epub 2015 May 22. PubMed
Covalent Modification of Bacteriophage T4 DNA Inhibits CRISPR-Cas9. Bryson AL, Hwang Y, Sherrill-Mix S, Wu GD, Lewis JD, Black L, Clark TA, Bushman FD. MBio. 2015 Jun 16;6(3):e00648. doi: 10.1128/mBio.00648-15. PubMed
CRISPR/Cas9-Mediated Re-Sensitization of Antibiotic-Resistant Escherichia coli Harboring Extended-Spectrum beta-Lactamases. Kim JS, Cho DH, Park M, Chung WJ, Shin D, Ko KS, Kweon DH. J Microbiol Biotechnol. 2016 Feb;26(2):394-401. doi: 10.4014/jmb.1508.08080. PubMed
Development of a CRISPR-Cas9 toolkit for comprehensive engineering of Bacillus subtilis. Westbrook AW, Moo-Young M, Chou CP. Appl Environ Microbiol. 2016 Jun 3. pii: AEM.01159-16. PubMed Associated Plasmids pAW005-2 pAW014-2 pAW016-2 pAW019-2
Enhanced integration of large DNA into E. coli chromosome by CRISPR/Cas9. Chung ME, Yeh IH, Sung LY, Wu MY, Chao YP, Ng IS, Hu YC. Biotechnol Bioeng. 2017 Jan;114(1):172-183. doi: 10.1002/bit.26056. Epub 2016 Aug 5. PubMed
CRISPR-Cas9 for the genome engineering of cyanobacteria and succinate production. Li H, Shen CR, Huang CH, Sung LY, Wu MY, Hu YC. Metab Eng. 2016 Nov;38:293-302. doi: 10.1016/j.ymben.2016.09.006. Epub 2016 Sep 28. PubMed
Investigation Into the Effects of Nucleotide Content on the Electrical Characteristics of DNA Plasmid Molecular Wires. Goshi N, Narenji A, Bui C, Mokili JL, Kassegne S. IEEE Trans Nanobioscience. 2016 Sep;15(6):585-594. PubMed
A CRISPR-Cas9 Assisted Non-Homologous End-Joining Strategy for One-step Engineering of Bacterial Genome. Su T, Liu F, Gu P, Jin H, Chang Y, Wang Q, Liang Q, Qi Q. Sci Rep. 2016 Nov 24;6:37895. doi: 10.1038/srep37895. PubMed
Development of a fast and easy method for Escherichia coli genome editing with CRISPR/Cas9. Zhao D, Yuan S, Xiong B, Sun H, Ye L, Li J, Zhang X, Bi C. Microb Cell Fact. 2016 Dec 1;15(1):205. PubMed
An Eight-Residue Deletion in Escherichia coli FabG Causes Temperature-Sensitive Growth and Lipid Synthesis Plus Resistance to the Calmodulin Inhibitor Trifluoperazine. Srinivas S, Cronan JE. J Bacteriol. 2017 Apr 25;199(10). pii: e00074-17. doi: 10.1128/JB.00074-17. Print 2017 May 15. PubMed
Genome Engineering of Virulent Lactococcal Phages Using CRISPR-Cas9. Lemay ML, Tremblay DM, Moineau S. ACS Synth Biol. 2017 Mar 30. doi: 10.1021/acssynbio.6b00388. PubMed Associated Plasmids pL2Cas9
Genome-Wide Abolishment of Mobile Genetic Elements Using Genome Shuffling and CRISPR/Cas-Assisted MAGE Allows the Efficient Stabilization of a Bacterial Chassis. Umenhoffer K, Draskovits G, Nyerges A, Karcagi I, Bogos B, Timar E, Csorgo B, Herczeg R, Nagy I, Feher T, Pal C, Posfai G. ACS Synth Biol. 2017 Apr 26. doi: 10.1021/acssynbio.6b00378. PubMed
The CRISPR/Cas9-facilitated multiplex pathway optimization (CFPO) technique and its application to improve the Escherichia coli xylose utilization pathway. Zhu X, Zhao D, Qiu H, Fan F, Man S, Bi C, Zhang X. Metab Eng. 2017 Aug 9;43(Pt A):37-45. doi: 10.1016/j.ymben.2017.08.003. PubMed
Combining CRISPR and CRISPRi Systems for Metabolic Engineering of E. coli and 1,4-BDO Biosynthesis. Wu MY, Sung LY, Li H, Huang CH, Hu YC. ACS Synth Biol. 2017 Dec 15;6(12):2350-2361. doi: 10.1021/acssynbio.7b00251. Epub 2017 Sep 12. PubMed
Efficient engineering of chromosomal ribosome binding site libraries in mismatch repair proficient Escherichia coli. Oesterle S, Gerngross D, Schmitt S, Roberts TM, Panke S. Sci Rep. 2017 Sep 26;7(1):12327. doi: 10.1038/s41598-017-12395-3. PubMed
Rapid and Scalable Characterization of CRISPR Technologies Using an E. coli Cell-Free Transcription-Translation System. Marshall R, Maxwell CS, Collins SP, Jacobsen T, Luo ML, Begemann MB, Gray BN, January E, Singer A, He Y, Beisel CL, Noireaux V. Mol Cell. 2018 Jan 4;69(1):146-157.e3. doi: 10.1016/j.molcel.2017.12.007. PubMed
Tailor-made gene silencing of Staphylococcus aureus clinical isolates by CRISPR interference. Sato'o Y, Hisatsune J, Yu L, Sakuma T, Yamamoto T, Sugai M. PLoS One. 2018 Jan 29;13(1):e0185987. doi: 10.1371/journal.pone.0185987. eCollection 2018. PubMed
CRISPR-based herd immunity can limit phage epidemics in bacterial populations. Payne P, Geyrhofer L, Barton NH, Bollback JP. Elife. 2018 Mar 9;7. pii: 32035. doi: 10.7554/eLife.32035. PubMed
Genome editing of Ralstonia eutropha using an electroporation-based CRISPR-Cas9 technique. Xiong B, Li Z, Liu L, Zhao D, Zhang X, Bi C. Biotechnol Biofuels. 2018 Jun 20;11:172. doi: 10.1186/s13068-018-1170-4. eCollection 2018. PubMed
Bacterial Adaptation to the Host's Diet Is a Key Evolutionary Force Shaping Drosophila-Lactobacillus Symbiosis. Martino ME, Joncour P, Leenay R, Gervais H, Shah M, Hughes S, Gillet B, Beisel C, Leulier F. Cell Host Microbe. 2018 Jul 11;24(1):109-119.e6. doi: 10.1016/j.chom.2018.06.001. Epub 2018 Jun 28. PubMed
Genome Editing with CRISPR-Cas9 in Lactobacillus plantarum Revealed That Editing Outcomes Can Vary Across Strains and Between Methods. Leenay RT, Vento JM, Shah M, Martino ME, Leulier F, Beisel CL. Biotechnol J. 2019 Mar;14(3):e1700583. doi: 10.1002/biot.201700583. Epub 2018 Sep 20. PubMed Associated Plasmids pCB578 pCB591
Multiple-step chromosomal integration of divided segments from a large DNA fragment via CRISPR/Cas9 in Escherichia coli. Li Y, Yan F, Wu H, Li G, Han Y, Ma Q, Fan X, Zhang C, Xu Q, Xie X, Chen N. J Ind Microbiol Biotechnol. 2019 Jan;46(1):81-90. doi: 10.1007/s10295-018-2114-5. Epub 2018 Nov 23. PubMed
Engineered K1F bacteriophages kill intracellular Escherichia coli K1 in human epithelial cells. Moller-Olsen C, Ho SFS, Shukla RD, Feher T, Sagona AP. Sci Rep. 2018 Dec 3;8(1):17559. doi: 10.1038/s41598-018-35859-6. PubMed
A rapid and versatile tool for genomic engineering in Lactococcus lactis. Guo T, Xin Y, Zhang Y, Gu X, Kong J. Microb Cell Fact. 2019 Jan 31;18(1):22. doi: 10.1186/s12934-019-1075-3. PubMed
Coupling ssDNA recombineering with CRISPR-Cas9 for Escherichia coli DnaG mutations. Li J, Sun J, Gao X, Wu Z, Shang G. Appl Microbiol Biotechnol. 2019 Apr;103(8):3559-3570. doi: 10.1007/s00253-019-09744-9. Epub 2019 Mar 16. PubMed
Development of a CRISPR/Cas9 System for Methylococcus capsulatus In Vivo Gene Editing. Tapscott T, Guarnieri MT, Henard CA. Appl Environ Microbiol. 2019 May 16;85(11). pii: AEM.00340-19. doi: 10.1128/AEM.00340-19. Print 2019 Jun 1. PubMed Associated Plasmids pCAH01SpR pCAH01Sp::Cas9 pCAH01Sp::Cas9D10A pQCH
Midbiotics: conjugative plasmids for genetic engineering of natural gut flora. Ruotsalainen P, Penttinen R, Mattila S, Jalasvuori M. Gut Microbes. 2019 Apr 5:1-11. doi: 10.1080/19490976.2019.1591136. PubMed
Construction of a bacterial surface display system based on outer membrane protein F. Chen T, Wang K, Chi X, Zhou L, Li J, Liu L, Zheng Q, Wang Y, Yu H, Gu Y, Zhang J, Li S, Xia N. Microb Cell Fact. 2019 Apr 11;18(1):70. doi: 10.1186/s12934-019-1120-2. PubMed
The phage T4 DNA ligase mediates bacterial chromosome DSBs repair as single component non-homologous end joining. Su T, Liu F, Chang Y, Guo Q, Wang J, Wang Q, Qi Q. Synth Syst Biotechnol. 2019 May 16;4(2):107-112. doi: 10.1016/j.synbio.2019.04.001. eCollection 2019 Jun. PubMed
Rapid Evolution of Reduced Susceptibility against a Balanced Dual-Targeting Antibiotic through Stepping-Stone Mutations. Szili P, Draskovits G, Revesz T, Bogar F, Balogh D, Martinek T, Daruka L, Spohn R, Vasarhelyi BM, Czikkely M, Kintses B, Grezal G, Ferenc G, Pal C, Nyerges A. Antimicrob Agents Chemother. 2019 Aug 23;63(9). pii: AAC.00207-19. doi: 10.1128/AAC.00207-19. Print 2019 Sep. PubMed
The Human Cytomegalovirus Chemokine vCXCL-1 Modulates Normal Dissemination Kinetics of Murine Cytomegalovirus In Vivo. Jackson JW, Hancock TJ, LaPrade E, Dogra P, Gann ER, Masi TJ, Panchanathan R, Miller WE, Wilhelm SW, Sparer TE. MBio. 2019 Jun 25;10(3). pii: mBio.01289-19. doi: 10.1128/mBio.01289-19. PubMed
Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis. Liao C, Ttofali F, Slotkowski RA, Denny SR, Cecil TD, Leenay RT, Keung AJ, Beisel CL. Nat Commun. 2019 Jul 3;10(1):2948. doi: 10.1038/s41467-019-10747-3. PubMed Associated Plasmids pCas9GG pFnCpf1GG pAsCpf1GG pLsCas13aGG pCas9FnCpf1GG More ...
CRISPR-Cas experiments for schools and the public. Ziegler H, Nellen W. Methods. 2020 Feb 1;172:86-94. doi: 10.1016/j.ymeth.2019.08.009. Epub 2019 Aug 28. PubMed
Modulation of Cas9 level for efficient CRISPR-Cas9-mediated chromosomal and plasmid gene deletion in Bacillus thuringiensis. Soonsanga S, Luxananil P, Promdonkoy B. Biotechnol Lett. 2020 Apr;42(4):625-632. doi: 10.1007/s10529-020-02809-0. Epub 2020 Jan 20. PubMed
Reversal of mcr-1-Mediated Colistin Resistance in Escherichia coli by CRISPR-Cas9 System. Wan P, Cui S, Ma Z, Chen L, Li X, Zhao R, Xiong W, Zeng Z. Infect Drug Resist. 2020 Apr 22;13:1171-1178. doi: 10.2147/IDR.S244885. eCollection 2020. PubMed
Phage-delivered sensitisation with subsequent antibiotic treatment reveals sustained effect against antimicrobial resistant bacteria. Liu H, Li H, Liang Y, Du X, Yang C, Yang L, Xie J, Zhao R, Tong Y, Qiu S, Song H. Theranostics. 2020 May 15;10(14):6310-6321. doi: 10.7150/thno.42573. eCollection 2020. PubMed
Beta-Lactam Sensitive Bacteria Can Acquire ESBL-Resistance via Conjugation after Long-Term Exposure to Lethal Antibiotic Concentration. Ruotsalainen P, Given C, Penttinen R, Jalasvuori M. Antibiotics (Basel). 2020 Jun 2;9(6). pii: antibiotics9060296. doi: 10.3390/antibiotics9060296. PubMed
A positive, growth-based PAM screen identifies noncanonical motifs recognized by the S. pyogenes Cas9. Collias D, Leenay RT, Slotkowski RA, Zuo Z, Collins SP, McGirr BA, Liu J, Beisel CL. Sci Adv. 2020 Jul 15;6(29):eabb4054. doi: 10.1126/sciadv.abb4054. eCollection 2020 Jul. PubMed
Phage-based biosensors: in vivo analysis of native T4 phage promoters to enhance reporter enzyme expression. Duong MM, Carmody CM, Nugen SR. Analyst. 2020 Sep 28;145(19):6291-6297. doi: 10.1039/d0an01413c. PubMed
A Novel White-to-Blue Colony Formation Assay to Select for Optimized sgRNAs. Wei C, Chen T, Zhang Y, Wang Y, Shi D, Jiang Z, Li K, Xiao L, Shen J. Mol Biotechnol. 2021 Jan;63(1):1-12. doi: 10.1007/s12033-020-00280-w. Epub 2020 Oct 12. PubMed
Optimization of T4 phage engineering via CRISPR/Cas9. Duong MM, Carmody CM, Ma Q, Peters JE, Nugen SR. Sci Rep. 2020 Oct 26;10(1):18229. doi: 10.1038/s41598-020-75426-6. PubMed
Manipulating the position of DNA expression cassettes using location tags fused to dCas9 (Cas9-Lag) to improve metabolic pathway efficiency. Xie Q, Li S, Zhao D, Ye L, Li Q, Zhang X, Zhu L, Bi C. Microb Cell Fact. 2020 Dec 14;19(1):229. doi: 10.1186/s12934-020-01496-w. PubMed
A multiplex CRISPR interference tool for virulence gene interrogation in Legionella pneumophila. Ellis NA, Kim B, Tung J, Machner MP. Commun Biol. 2021 Feb 4;4(1):157. doi: 10.1038/s42003-021-01672-7. PubMed
An Intronic Risk SNP rs12454712 for Central Obesity Acts As an Allele-Specific Enhancer To Regulate BCL2 Expression. Dong SS, Zhu DL, Zhou XR, Rong Y, Zeng M, Chen JB, Jiang F, Tuo XM, Feng Z, Yang TL, Guo Y. Diabetes. 2021 Aug;70(8):1679-1688. doi: 10.2337/db20-1151. Epub 2021 May 25. PubMed
Role of AmpG in the resistance to beta-lactam agents, including cephalosporins and carbapenems: candidate for a novel antimicrobial target. D'Souza R, Nguyen LP, Pinto NA, Lee H, Vu TN, Kim H, Cho HS, Yong D. Ann Clin Microbiol Antimicrob. 2021 Jun 16;20(1):45. doi: 10.1186/s12941-021-00446-7. PubMed
Analysing Parallel Strategies to Alter the Host Specificity of Bacteriophage T7. Avramucz A, Moller-Olsen C, Grigonyte AM, Paramalingam Y, Millard A, Sagona AP, Feher T. Biology (Basel). 2021 Jun 20;10(6). pii: biology10060556. doi: 10.3390/biology10060556. PubMed
Wobble Editing of Cre-box by Unspecific CRISPR/Cas9 Causes CCR Release and Phenotypic Changes in Bacillus pumilus. Wang Y, Cao L, Bi M, Wang S, Chen M, Chen X, Ying M, Huang L. Front Chem. 2021 Aug 9;9:717609. doi: 10.3389/fchem.2021.717609. eCollection 2021. PubMed
Targeted chromosomal Escherichia coli:dnaB exterior surface residues regulate DNA helicase behavior to maintain genomic stability and organismal fitness. Behrmann MS, Perera HM, Hoang JM, Venkat TA, Visser BJ, Bates D, Trakselis MA. PLoS Genet. 2021 Nov 12;17(11):e1009886. doi: 10.1371/journal.pgen.1009886. eCollection 2021 Nov. PubMed
A Strategy for the Rapid Development of a Safe Vibrio cholerae Candidate Vaccine Strain. Karpov DS, Goncharenko AV, Usachev EV, Vasina DV, Divisenko EV, Chalenko YM, Pochtovyi AA, Ovchinnikov RS, Makarov VV, Yudin SM, Tkachuk AP, Gushchin VA. Int J Mol Sci. 2021 Oct 28;22(21). pii: ijms222111657. doi: 10.3390/ijms222111657. PubMed
Alteration of Salmonella enterica Virulence and Host Pathogenesis through Targeting sdiA by Using the CRISPR-Cas9 System. Askoura M, Almalki AJ, Lila ASA, Almansour K, Alshammari F, Khafagy ES, Ibrahim TS, Hegazy WAH. Microorganisms. 2021 Dec 11;9(12). pii: microorganisms9122564. doi: 10.3390/microorganisms9122564. PubMed
inPOSE: A Flexible Toolbox for Chromosomal Cloning and Amplification of Bacterial Transgenes. Shukla RD, Zvara A, Avramucz A, Biketova AY, Nyerges A, Puskas LG, Feher T. Microorganisms. 2022 Jan 21;10(2):236. doi: 10.3390/microorganisms10020236. PubMed
CRISPR/cas9 cassette targeting Escherichia coli (bla)CTX-M specific gene of mastitis cow milk origin can alter the antibiotic resistant phenotype for cefotaxime. Deb R, Chaudhary P, De S. Anim Biotechnol. 2022 Mar 31:1-6. doi: 10.1080/10495398.2022.2053695. PubMed
An Osteoporosis Susceptibility Allele at 11p15 Regulates SOX6 Expression by Modulating TCF4 Chromatin Binding. Zhu DL, Chen XF, Zhou XR, Hu SY, Tuo XM, Hao RH, Dong SS, Jiang F, Rong Y, Yang TL, Yang Z, Guo Y. J Bone Miner Res. 2022 Jun;37(6):1147-1155. doi: 10.1002/jbmr.4554. Epub 2022 Apr 17. PubMed
Phage anti-CBASS and anti-Pycsar nucleases subvert bacterial immunity. Hobbs SJ, Wein T, Lu A, Morehouse BR, Schnabel J, Leavitt A, Yirmiya E, Sorek R, Kranzusch PJ. Nature. 2022 May;605(7910):522-526. doi: 10.1038/s41586-022-04716-y. Epub 2022 Apr 8. PubMed
CRISPR/Cas9-Based Deletion of SpvB Gene From Salmonella gallinarum Leads to Loss of Virulence in Chicken. Basit A, Tahir H, Haider Z, Tariq H, Ullah A, Rehman SU. Front Bioeng Biotechnol. 2022 Jun 13;10:885227. doi: 10.3389/fbioe.2022.885227. eCollection 2022. PubMed
CRISPR-Cas9 Based Bacteriophage Genome Editing. Zhang X, Zhang C, Liang C, Li B, Meng F, Ai Y. Microbiol Spectr. 2022 Aug 31;10(4):e0082022. doi: 10.1128/spectrum.00820-22. Epub 2022 Jul 26. PubMed
A Polyketide Synthetase Gene Cluster Is Responsible for Antibacterial Activity of Burkholderia contaminans MS14. Deng P, Jia J, Foxfire A, Baird SM, Smith LJ, Lu SE. Phytopathology. 2023 Jan;113(1):11-20. doi: 10.1094/PHYTO-03-22-0106-R. Epub 2023 Jan 13. PubMed
The evolution of a counter-defense mechanism in a virus constrains its host range. Srikant S, Guegler CK, Laub MT. Elife. 2022 Aug 4;11:e79549. doi: 10.7554/eLife.79549. PubMed
Resensitization of Fosfomycin-Resistant Escherichia coli Using the CRISPR System. Walflor HSM, Lucena ARC, Tuon FF, Medeiros LCS, Faoro H. Int J Mol Sci. 2022 Aug 16;23(16):9175. doi: 10.3390/ijms23169175. PubMed
The Role of O-antigen in P1 Transduction of Shigella flexneri and Escherichia coli with its Alternative S' Tail Fibre. Huan YW, Fa-Arun J, Wang B. J Mol Biol. 2022 Sep 15;434(21):167829. doi: 10.1016/j.jmb.2022.167829. PubMed Associated Plasmids 4A3.SU 4A3.S'U'
CRISPR-based engineering of phages for in situ bacterial base editing. Nethery MA, Hidalgo-Cantabrana C, Roberts A, Barrangou R. Proc Natl Acad Sci U S A. 2022 Nov 16;119(46):e2206744119. doi: 10.1073/pnas.2206744119. Epub 2022 Nov 7. PubMed
Self-assembled endogenous DNA nanoparticles for auto-release and expression of the eGFP gene in Bacillus subtilis. Cao L, Meng Z, Tan J, Ying M, Bi M, Liu Y, Tong X, Wei J, Huang L. Commun Biol. 2022 Dec 14;5(1):1373. doi: 10.1038/s42003-022-04233-8. PubMed
Coupling CRISPR/Cas9 and Lambda Red Recombineering System for Genome Editing of Salmonella Gallinarum and the Effect of ssaU Knock-Out Mutant on the Virulence of Bacteria. Tahir H, Basit A, Tariq H, Haider Z, Ullah A, Hayat Z, Rehman SU. Biomedicines. 2022 Nov 24;10(12):3028. doi: 10.3390/biomedicines10123028. PubMed
Assessment of stromal SCD-induced drug resistance of PDAC using 3D-printed zPDX model chips. Wu C, Hu B, Wang L, Wu X, Gu H, Dong H, Yan J, Qi Z, Zhang Q, Chen H, Yu B, Hu S, Qian Y, Dong S, Li Q, Wang X, Long J. iScience. 2022 Dec 5;26(1):105723. doi: 10.1016/j.isci.2022.105723. eCollection 2023 Jan 20. PubMed
Molecular mechanism of lipopolysaccharide (LPS) in promoting biomineralization on bacterial surface. Gong Z, Guo J, Li Q, Xie H. Biochim Biophys Acta Gen Subj. 2023 Mar;1867(3):130305. doi: 10.1016/j.bbagen.2023.130305. Epub 2023 Jan 5. PubMed
Systematically attenuating DNA targeting enables CRISPR-driven editing in bacteria. Collias D, Vialetto E, Yu J, Co K, Almasi EDH, Ruttiger AS, Achmedov T, Strowig T, Beisel CL. Nat Commun. 2023 Feb 8;14(1):680. doi: 10.1038/s41467-023-36283-9. PubMed
P1 Bacteriophage-Enabled Delivery of CRISPR-Cas9 Antimicrobial Activity Against Shigella flexneri. Huan YW, Torraca V, Brown R, Fa-Arun J, Miles SL, Oyarzun DA, Mostowy S, Wang B. ACS Synth Biol. 2023 Mar 17;12(3):709-721. doi: 10.1021/acssynbio.2c00465. Epub 2023 Feb 20. PubMed Associated Plasmids pBBR1.cas9-NT
Ubiquitin-like conjugation by bacterial cGAS enhances anti-phage defence. Jenson JM, Li T, Du F, Ea CK, Chen ZJ. Nature. 2023 Apr;616(7956):326-331. doi: 10.1038/s41586-023-05862-7. Epub 2023 Feb 27. PubMed
A swapped genetic code prevents viral infections and gene transfer. Nyerges A, Vinke S, Flynn R, Owen SV, Rand EA, Budnik B, Keen E, Narasimhan K, Marchand JA, Baas-Thomas M, Liu M, Chen K, Chiappino-Pepe A, Hu F, Baym M, Church GM. Nature. 2023 Mar;615(7953):720-727. doi: 10.1038/s41586-023-05824-z. Epub 2023 Mar 15. PubMed
Enhancer variants on chromosome 2p14 regulating SPRED2 and ACTR2 act as a signal amplifier to protect against rheumatoid arthritis. Wang NN, Zhang Y, Jiang F, Zhu DL, Di CX, Hu SY, Chen XF, Zhi LQ, Rong Y, Ke X, Duan YY, Dong SS, Yang TL, Yang Z, Guo Y. Am J Hum Genet. 2023 Apr 6;110(4):625-637. doi: 10.1016/j.ajhg.2023.02.012. Epub 2023 Mar 15. PubMed
Degradation of Antibiotic Resistance Genes by VADER with CRISPR-Cas Immunity. Li X, Bao N, Yan Z, Yuan XZ, Wang SG, Xia PF. Appl Environ Microbiol. 2023 Apr 26;89(4):e0005323. doi: 10.1128/aem.00053-23. Epub 2023 Mar 28. PubMed
Enhanced production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with modulated 3-hydroxyvalerate fraction by overexpressing acetolactate synthase in Cupriavidus necator H16. Jo YY, Park S, Gong G, Roh S, Yoo J, Ahn JH, Lee SM, Um Y, Kim KH, Ko JK. Int J Biol Macromol. 2023 Jul 1;242(Pt 4):125166. doi: 10.1016/j.ijbiomac.2023.125166. Epub 2023 Jun 2. PubMed
Characterization of piggery farm waste-borne bacterial transposable elements associated with antimicrobial resistance phenotypes. Thakur PK, Deb R, Pegu SR, Parihar R, Niharika J, Jyoti Das P, Sengar GS, Sonowal J, Chaudhary P, Selvaradjou A, Raj A, Gupta VK. Comp Immunol Microbiol Infect Dis. 2023 Jul;98:102005. doi: 10.1016/j.cimid.2023.102005. Epub 2023 Jun 14. PubMed
Engineered reporter phages for detection of Escherichia coli, Enterococcus, and Klebsiella in urine. Meile S, Du J, Staubli S, Grossmann S, Koliwer-Brandl H, Piffaretti P, Leitner L, Matter CI, Baggenstos J, Hunold L, Milek S, Guebeli C, Kozomara-Hocke M, Neumeier V, Botteon A, Klumpp J, Marschall J, McCallin S, Zbinden R, Kessler TM, Loessner MJ, Dunne M, Kilcher S. Nat Commun. 2023 Jul 20;14(1):4336. doi: 10.1038/s41467-023-39863-x. PubMed
Monomeric streptavidin phage display allows efficient immobilization of bacteriophages on magnetic particles for the capture, separation, and detection of bacteria. Carmody CM, Nugen SR. Sci Rep. 2023 Sep 27;13(1):16207. doi: 10.1038/s41598-023-42626-9. PubMed
Elimination of bla(KPC-2)-mediated carbapenem resistance in Escherichia coli by CRISPR-Cas9 system. Tao S, Chen H, Li N, Fang Y, Zhang H, Xu Y, Chen L, Liang W. BMC Microbiol. 2023 Oct 26;23(1):310. doi: 10.1186/s12866-023-03058-7. PubMed
Targeted elimination of Vancomycin resistance gene vanA by CRISPR-Cas9 system. Tao S, Hu C, Fang Y, Zhang H, Xu Y, Zheng L, Chen L, Liang W. BMC Microbiol. 2023 Dec 4;23(1):380. doi: 10.1186/s12866-023-03136-w. PubMed
CRISPR-COPIES: an in silico platform for discovery of neutral integration sites for CRISPR/Cas-facilitated gene integration. Boob AG, Zhu Z, Intasian P, Jain M, Petrov VA, Lane ST, Tan SI, Xun G, Zhao H. Nucleic Acids Res. 2024 Apr 12;52(6):e30. doi: 10.1093/nar/gkae062. PubMed
CRISPR/Cpf1-FOKI-induced gene editing in Gluconobacter oxydans. Wang X, Li D, Qin Z, Chen J, Zhou J. Synth Syst Biotechnol. 2024 Mar 4;9(2):369-379. doi: 10.1016/j.synbio.2024.02.009. eCollection 2024 Jun. PubMed
Systematic interrogation of CRISPR antimicrobials in Klebsiella pneumoniae reveals nuclease-, guide- and strain-dependent features influencing antimicrobial activity. Vialetto E, Miele S, Goren MG, Yu J, Yu Y, Collias D, Beamud B, Osbelt L, Lourenco M, Strowig T, Brisse S, Barquist L, Qimron U, Bikard D, Beisel CL. Nucleic Acids Res. 2024 Jun 10;52(10):6079-6091. doi: 10.1093/nar/gkae281. PubMed

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