pcDNA3-3myc-6His-EZH2 21A
(Plasmid #42663)

• Depositing Lab: Mien-Chie Hung
• Publication: Cha et al Science. 2005 Oct 14;310(5746):306-10.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5446
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EZH2
• Alt name: enhancer of zeste homolog 2
• Species: H. sapiens (human)
• Insert Size (bp): 2400
• Mutation: S21A
• Entrez Gene: EZH2 (a.k.a. ENX-1, ENX1, EZH2b, KMT6, KMT6A, WVS, WVS2)
• Tags / Fusion Proteins:
  • 3Myc (N terminal on insert)
  • 6His (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: A. Chinnaiyan
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that there is an additional mutation in EZH2 ORF, Q3R. The mutation does not affect plasmid function.