hu-TLR4 399 SNP flag pLenti4/To/v5/DEST
(Plasmid #42651)

• Purpose: destination vector for lentivirus-mediated expression of human TLR4 399 SNP in mammalian cells
• Depositing Lab: Scott Friedman
• Publication: Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 10.1002/hep.22697.

Backbone

• Vector backbone: Plenti4/TO/V5-DEST
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 8634
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TLR4 399 SNP
• Alt name: toll-like receptor 4
• Alt name: TLR4
• Species: H. sapiens (human)
• Mutation: T399I
• Entrez Gene: TLR4 (a.k.a. ARMD10, CD284, TLR-4, TOLL)
• Promoter: CMV/TO
• Tag / Fusion Protein:
  • Flag (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: pBabe 3'

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry