hu-TLR4 299 SNP flag pLenti 4/to/v5 pDEST
(Plasmid
#42650)
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Purposedestination vector for lentivirus-mediated expression of human TLR4 299 SNP in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 42650 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePlenti4/TO/V5-DEST
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 8634
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Vector typeMammalian Expression, Lentiviral
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTLR4 299 SNP
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Alt nametoll-like receptor 4
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Alt nameTLR4
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SpeciesH. sapiens (human)
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MutationD299G
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Entrez GeneTLR4 (a.k.a. ARMD10, CD284, TLR-4, TOLL)
- Promoter CMV/TO
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Tag
/ Fusion Protein
- Flag (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer pBabe 3' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
hu-TLR4 299 SNP flag pLenti 4/to/v5 pDEST was a gift from Scott Friedman (Addgene plasmid # 42650 ; http://n2t.net/addgene:42650 ; RRID:Addgene_42650) -
For your References section:
Functional linkage of cirrhosis-predictive single nucleotide polymorphisms of Toll-like receptor 4 to hepatic stellate cell responses. Guo J, Loke J, Zheng F, Hong F, Yea S, Fukata M, Tarocchi M, Abar OT, Huang H, Sninsky JJ, Friedman SL. Hepatology. 2009 Mar;49(3):960-8. doi: 10.1002/hep.22697. 10.1002/hep.22697 PubMed 19085953
