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hu-TLR4 299/399 dual snp flag pDEST40
(Plasmid #42648)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42648 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA DEST40
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 7143
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TLR4 299/399 SNP
  • Alt name
    toll-like receptor 4
  • Alt name
    TLR4
  • Species
    H. sapiens (human)
  • Mutation
    D299G and T399I
  • Entrez Gene
    TLR4 (a.k.a. ARMD10, CD284, TLR-4, TOLL)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hu-TLR4 299/399 dual snp flag pDEST40 was a gift from Scott Friedman (Addgene plasmid # 42648 ; http://n2t.net/addgene:42648 ; RRID:Addgene_42648)

  • For your References section:

    Functional linkage of cirrhosis-predictive single nucleotide polymorphisms of Toll-like receptor 4 to hepatic stellate cell responses. Guo J, Loke J, Zheng F, Hong F, Yea S, Fukata M, Tarocchi M, Abar OT, Huang H, Sninsky JJ, Friedman SL. Hepatology. 2009 Mar;49(3):960-8. doi: 10.1002/hep.22697. 10.1002/hep.22697 PubMed 19085953
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