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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 42633 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonePet28a
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5368
- Total vector size (bp) 5557
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Modifications to backboneNone
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsNone
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameHuman H4
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SpeciesH. sapiens (human)
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Insert Size (bp)312
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GenBank IDNP_003529.1
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Entrez GeneH4C1 (a.k.a. H4-16, H4C11, H4C12, H4C13, H4C14, H4C15, H4C16, H4C2, H4C3, H4C4, H4C5, H4C6, H4C8, H4C9, H4FA, HIST1H4A)
- Promoter T7
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Tag
/ Fusion Protein
- Untagged
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T7 Promoter
- 3′ sequencing primer T7 Terminator (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Pet28a_Human_H4 was a gift from Joe Landry (Addgene plasmid # 42633 ; http://n2t.net/addgene:42633 ; RRID:Addgene_42633)