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Pet28a_Human_H3.3
(Plasmid #42632)

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Item Catalog # Description Quantity Price (USD)
Plasmid 42632 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    Pet28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5368
  • Total vector size (bp) 5656
  • Modifications to backbone
    None
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    None
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Human H3.3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    411
  • GenBank ID
    NP_002098.1
  • Entrez Gene
    H3-3A (a.k.a. BRYLIB1, H3-3B, H3.3A, H3F3, H3F3A)
  • Promoter T7
  • Tag / Fusion Protein
    • Untagged

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer T7 Promoter
  • 3′ sequencing primer T7 Terminator
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Pet28a_Human_H3.3 was a gift from Joe Landry (Addgene plasmid # 42632 ; http://n2t.net/addgene:42632 ; RRID:Addgene_42632)
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