pcDNA3-APX(W41F)-NES
(Plasmid #42608)

• Depositing Lab: Alice Ting
• Publication: Rhee et al Science. 2013 Jan 31.

Backbone

• Vector backbone: pcDNA3
• Total vector size (bp): 6198
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: soybean APX W41F (dimeric) -NES
• Species: soybean
• Insert Size (bp): 810
• Mutation: W41F mutation
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMVf

Resource Information

• Supplemental Documents:
  • APEX2 Recommendations from Ting Lab

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is for expression of soybean APX W41F (dimeric) in mammalian cells under the CMV promoter. In this construct, APX has a C-terminal nuclear export signal (NES), with amino acid sequence LQLPPLERLTLD.