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Addgene

pGC480
(Plasmid #42600)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42600 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    L4440
  • Backbone size w/o insert (bp) 2790
  • Total vector size (bp) 3838
  • Vector type
    RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Tetracycline, 100 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Grow on LB + Amp + Tet plates. For RNAi feeding of worms grow LB liquid cultures 16 hours with only Amp.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    let-363 cDNA
  • gRNA/shRNA sequence
    let-363
  • Species
    C. elegans (nematode)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer M13F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid can be kept in DH5alpha cells but does not work for RNAi in that strain. For RNAi, please transform DNA into the HT115 strain.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGC480 was a gift from Jane Hubbard (Addgene plasmid # 42600 ; http://n2t.net/addgene:42600 ; RRID:Addgene_42600)

  • For your References section:

    S6K links cell fate, cell cycle and nutrient response in C. elegans germline stem/progenitor cells. Korta DZ, Tuck S, Hubbard EJ. Development. 2012 Mar;139(5):859-70. doi: 10.1242/dev.074047. Epub 2012 Jan 25. 10.1242/dev.074047 PubMed 22278922
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