phpRL-BCL2-FL-pA
(Plasmid #42595)

• Depositing Lab: Richard Lloyd
• Publication: Sherrill et al J Biol Chem. 2004 Jul 9;279(28):29066-74. Epub 2004 May 3.

Backbone

• Vector backbone: pRL-HL
• Backbone manufacturer: Stanley Lemon (PubMed ID 10611164)
• Total vector size (bp): 9596
• Vector type: Mammalian Expression, Luciferase
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hairpin - renilla luciferase - BCL2 5'UTR - firefly luciferase
• Species: H. sapiens (human)
• Insert Size (bp): 3900
• Promoter: CMV

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

phpRL-BCL2-FL(pA) includes a 143-bp stable RNA hairpin located upstream of renilla luciferase, BCL2 5'UTR and firefly luciferase. See plasmid map link above for more details.

This plasmid is one of several reporter constructs created to assess potential IRES activity of the BCL2 5'UTR. The hairpin in this construct inhibits translation of renilla luciferase and to a lesser extent translation of firefly luciferase.

Please note that Addgene's sequencing results differ from the full plasmid sequence provided by the depositing laboratory. These differences do not affect the function of the plasmid.