pAAV_TALEN-FokI(WT)-BB_V3
(Plasmid #42588)

• Depositing Lab: Feng Zhang
• Publication: Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962.

Backbone

• Vector backbone: pAAV
• Total vector size (bp): 6542
• Vector type: Mammalian Expression, AAV ; Adeno-associated Virus ()

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Growth instructions: Backbone contains ccdb gene, serve as a negative selection cassette. Please use ccdb resistant strain for maintenance of the plasmid.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TALEN-FokI(WT)-BB
• Alt name: TALEN Backbone
• Alt name: TALE Nuclease
• Alt name: TALEN FokI
• Promoter: CAG hybrid
• Tag / Fusion Protein:
  • 3XFlag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI/BamHI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: CAG-forward
• 3′ sequencing primer: BGH-reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is a new collection of constructs described in Cong et al. Nature Communications and also an updated TALE Tool Box containing Adeno-associated Virus (AAV) TALE Backbone constructs that allow packaging of TAL transcription factors (TALE-TF) and TALE nucleases (TALEN) into AAV. All constructs are from Feng Zhang lab at Broad Institute/MIT.

IMPORTANT: please note when using the new AAV TALE Backbones, the last 0.5 (half) repeat is not encoded in the backbone vector but instead need to be supplemented as a separate piece.