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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 42581 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
- Total vector size (bp) 6119
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Vector typeMammalian Expression, AAV ; Adeno-associated Virus ()
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Growth instructionsBackbone contains ccdb gene, serve as a negative selection cassette. Please use ccdb resistant strain for maintenance of the plasmid.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTALE-TF(VP64)-BB
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Alt nameTALE Backbone
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Alt nameTALE VP64
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Insert Size (bp)2015
- Promoter CAG hybrid
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Tag
/ Fusion Protein
- 3XFlag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI/BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CAG-forward
- 3′ sequencing primer BGH-reverse (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This is a new collection of constructs described in Cong et al. Nature Communications and also an updated TALE Tool Box containing Adeno-associated Virus (AAV) TALE Backbone constructs that allow packaging of TAL transcription factors (TALE-TF) and TALE nucleases (TALEN) into AAV. All constructs are from Feng Zhang lab at Broad Institute/MIT.
IMPORTANT: please note when using the new AAV TALE Backbones, the last 0.5 (half) repeat is not encoded in the backbone vector but instead need to be supplemented as a separate piece.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV_TALE-TF(VP64)-BB_V3 was a gift from Feng Zhang (Addgene plasmid # 42581 ; http://n2t.net/addgene:42581 ; RRID:Addgene_42581) -
For your References section:
Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains. Cong L, Zhou R, Kuo YC, Cunniff M, Zhang F. Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962. 10.1038/ncomms1962 PubMed 22828628