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Addgene

pNMT
(Plasmid #42578)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42578 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSF-1
  • Backbone manufacturer
    Novagen/EMD Millipore
  • Backbone size w/o insert (bp) 3600
  • Total vector size (bp) 4968
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    N-myristoyl tranferase
  • Alt name
    NMT
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1368
  • Mutation
    two silent mutations T690C and T942C
  • GenBank ID
    M23726.1
  • Entrez Gene
    NMT1 (a.k.a. YLR195C, CDC72)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (unknown if destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Used for production of myristylated HIV-1 Gag in E. coli.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNMT was a gift from James Hurley (Addgene plasmid # 42578 ; http://n2t.net/addgene:42578 ; RRID:Addgene_42578)

  • For your References section:

    In vitro reconstitution of the ordered assembly of the endosomal sorting complex required for transport at membrane-bound HIV-1 Gag clusters. Carlson LA, Hurley JH. Proc Natl Acad Sci U S A. 2012 Oct 16;109(42):16928-33. doi: 10.1073/pnas.1211759109. Epub 2012 Oct 1. 10.1073/pnas.1211759109 PubMed 23027949
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