pHisAlix
(Plasmid #42576)

• Depositing Lab: James Hurley
• Publication: Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;109(42):16928-33. doi: 10.1073/pnas.1211759109. Epub 2012 Oct 1.

Backbone

• Vector backbone: pHIS-parallel2
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 8191
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ALIX
• Alt name: isoform 1, lacks 5 amino acids present in isoform 2
• Species: H. sapiens (human)
• Insert Size (bp): 2691
• Mutation: silent mutation A1617G (numbering in ALIX gene)
• GenBank ID: NM_013374.4
• Entrez Gene: PDCD6IP (a.k.a. AIP1, ALIX, DRIP4, HP95, MCPH29)
• Tag / Fusion Protein:
  • HIS-TEV (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry