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pEXP5-NT-7th R
(Plasmid #42573)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42573 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEXP5-NT/TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2745
  • Total vector size (bp) 3194
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mutated calmodulin gene
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    449
  • Mutation
    changed Phenylalanine 92 to Glutamine acid, Isoleucine 85 to Leucine, Alanine 88 to Glutamine, Histidine 107 to Isoleucine, Leucine 112 to Arginine, Methionine 124 to Leucine, Alanine 128 to Threonine, Methionine 144 to Arginine
  • Promoter T7
  • Tag / Fusion Protein
    • Polyhistidine (6×His) region, TEV recognition site (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEXP5-NT-7th R was a gift from Ivan Korendovych (Addgene plasmid # 42573 ; http://n2t.net/addgene:42573 ; RRID:Addgene_42573)

  • For your References section:

    A Single Mutation in a Regulatory Protein Produces Evolvable Allosterically Regulated Catalyst of Nonnatural Reaction. Moroz OV, Moroz YS, Wu Y, Olsen AB, Cheng H, Mack KL, McLaughlin JM, Raymond EA, Zhezherya K, Roder H, Korendovych IV. Angew Chem Int Ed Engl. 2013 Apr 29. doi: 10.1002/anie.201302339. 10.1002/anie.201302339 PubMed 23630096
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