pDEST17-Tpt1
(Plasmid
#42571)
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Depositing Labs
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 42571 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDEST17
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Backbone manufacturerInvitrogen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTpt1
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SpeciesS. cerevisiae (budding yeast)
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GenBank IDCAA99116.1
- Promoter T7 RNA Polmyerase
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Tag
/ Fusion Protein
- 6xHistidine (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7 Promoter
- 3′ sequencing primer T7 Terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See publication for expression protocol. Expressed in BL21 (DE3) RIPLE cells from Stratagene. Highly expressed and soluble.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDEST17-Tpt1 was a gift from Stan Fields & Jay Hesselberth (Addgene plasmid # 42571 ; http://n2t.net/addgene:42571 ; RRID:Addgene_42571) -
For your References section:
Capture and sequence analysis of RNAs with terminal 2',3'-cyclic phosphates. Schutz K, Hesselberth JR, Fields S. RNA. 2010 Mar;16(3):621-31. Epub 2010 Jan 14. 10.1261/rna.1934910 PubMed 20075163