Skip to main content
Addgene

pCMV-Pam-FL
(Plasmid #42570)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42570 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCMV-3B
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4300
  • Total vector size (bp) 19009
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    human MYCBP2
  • Alt name
    PAM
  • Alt name
    Protein Associated with Myc
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    14800
  • Mutation
    Pam-FL clone lacks alternatively spliced exons 52A, 53, 56A (central Myc-binding region)
  • GenBank ID
  • Entrez Gene
    MYCBP2 (a.k.a. Myc-bp2, PAM, PHR1, Phr)
  • Promoter CMV
  • Tag / Fusion Protein
    • Myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (destroyed during cloning)
  • 3′ cloning site EcoRV (destroyed during cloning)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    V. Ramesh lab

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene QC NGS analysis identified sequence discrepancies relative to NM_015057. Please also see the associated publication for additional information on the construction of this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-Pam-FL was a gift from Vijaya Ramesh (Addgene plasmid # 42570 ; http://n2t.net/addgene:42570 ; RRID:Addgene_42570)

  • For your References section:

    Alternative splicing in protein associated with Myc (Pam) influences its binding to c-Myc. Santos TM, Han S, Bowser M, Sazani K, Beauchamp RL, Murthy V, Bhide PG, Ramesh V. J Neurosci Res. 2006 Feb 1;83(2):222-32. 10.1002/jnr.20723 PubMed 16342205
Related items:
Commonly requested with: