pCMV-Pam-FL
(Plasmid #42570)

• Depositing Lab: Vijaya Ramesh
• Publication: Santos et al J Neurosci Res. 2006 Feb 1;83(2):222-32.

Backbone

• Vector backbone: pCMV-3B
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 4300
• Total vector size (bp): 19009
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: human MYCBP2
• Alt name: PAM
• Alt name: Protein Associated with Myc
• Species: H. sapiens (human)
• Insert Size (bp): 14800
• Mutation: Pam-FL clone lacks alternatively spliced exons 52A, 53, 56A (central Myc-binding region)
• GenBank ID:
• Entrez Gene: MYCBP2 (a.k.a. Myc-bp2, PAM, PHR1, Phr)
• Promoter: CMV
• Tag / Fusion Protein:
  • Myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (destroyed during cloning)
• 3′ cloning site: EcoRV (destroyed during cloning)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: V. Ramesh lab

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene QC NGS analysis identified sequence discrepancies relative to NM_015057. Please also see the associated publication for additional information on the construction of this plasmid.