pEXP5-NT-CuSeCat EE
(Plasmid #42569)

• Depositing Lab: Ivan Korendovych
• Publication: Mack et al J Biol Inorg Chem. 2013 Apr;18(4):411-8. doi: 10.1007/s00775-013-0985-5. Epub 2013 Feb 19.

Backbone

• Vector backbone: pEXP5-NT/TOPO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2745
• Total vector size (bp): 3194
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mutated calmodulin gene
• Species: G. gallus (chicken)
• Insert Size (bp): 449
• Mutation: changed Phenylalanine 92 to Glutamine acid, Valine 91 to Methionine, Serine 101 to Glutamine acid, Histidine 107 to Isoleucine, Leucine 112 to Arginine, Asparagine 137 to Glutamine acid, Methionine 144 to Arginine, Threonine 146 to Arginine
• Promoter: T7
• Tag / Fusion Protein:
  • Polyhistidine (6×His) region, TEV recognition site (N terminal on insert)

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry