pEXP5-NT-CuSeCat
(Plasmid #42568)

• Depositing Lab: Ivan Korendovych
• Publication: Mack et al J Biol Inorg Chem. 2013 Apr;18(4):411-8. doi: 10.1007/s00775-013-0985-5. Epub 2013 Feb 19.

Backbone

• Vector backbone: pEXP5-NT/TOPO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2745
• Total vector size (bp): 3194
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mutated calmodulin gene
• Species: G. gallus (chicken)
• Insert Size (bp): 449
• Mutation: changed Phenylalanine 92 to Glutamine acid, Valine 91 to Methionine, Histidine 107 to Isoleucine, Leucine 112 to Arginine, Methionine 144 to Arginine, Threonine 146 to Arginine
• Promoter: T7
• Tag / Fusion Protein:
  • Polyhistidine (6×His) region, TEV recognition site (N terminal on insert)

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry