myc ATG18
(Plasmid
#42530)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 42530 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneYcplac111
- Backbone size w/o insert (bp) 6112
- Total vector size (bp) 8228
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameATG18
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)2116
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Mutationnone
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Entrez GeneATG18 (a.k.a. YFR021W, AUT10, CVT18, NMR1, SVP1)
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Tag
/ Fusion Protein
- myc (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer m13 forward
- 3′ sequencing primer m13 reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
ATG18 was cloned with 300bp upstream and downstream of the gene. The myc tag was added later by mutagenesis.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
myc ATG18 was a gift from James Hurley (Addgene plasmid # 42530 ; http://n2t.net/addgene:42530 ; RRID:Addgene_42530) -
For your References section:
Two-site recognition of phosphatidylinositol 3-phosphate by PROPPINs in autophagy. Baskaran S, Ragusa MJ, Boura E, Hurley JH. Mol Cell. 2012 Aug 10;47(3):339-48. doi: 10.1016/j.molcel.2012.05.027. Epub 2012 Jun 14. 10.1016/j.molcel.2012.05.027 PubMed 22704557