pJK59-ATG18-GFP
(Plasmid #42529)

• Depositing Lab: James Hurley
• Publication: Baskaran et al Mol Cell. 2012 Aug 10;47(3):339-48. doi: 10.1016/j.molcel.2012.05.027. Epub 2012 Jun 14.

Backbone

• Vector backbone: pRS316
• Backbone size w/o insert (bp): 5756
• Total vector size (bp): 7556
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Atg18
• Alt name: svp1
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1800
• Mutation: none
• Entrez Gene: ATG18 (a.k.a. YFR021W, AUT10, CVT18, NMR1, SVP1)
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: internal primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: the plasmid was obtained from Will Prinz

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid was constructed by amplifying Atg18 with 300bp 5' of the start site.