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Addgene

pJK59-ATG18-GFP
(Plasmid #42529)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42529 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS316
  • Backbone size w/o insert (bp) 5756
  • Total vector size (bp) 7556
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Atg18
  • Alt name
    svp1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1800
  • Mutation
    none
  • Entrez Gene
    ATG18 (a.k.a. YFR021W, AUT10, CVT18, NMR1, SVP1)
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer internal primer
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    the plasmid was obtained from Will Prinz

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid was constructed by amplifying Atg18 with 300bp 5' of the start site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK59-ATG18-GFP was a gift from James Hurley (Addgene plasmid # 42529 ; http://n2t.net/addgene:42529 ; RRID:Addgene_42529)

  • For your References section:

    Two-site recognition of phosphatidylinositol 3-phosphate by PROPPINs in autophagy. Baskaran S, Ragusa MJ, Boura E, Hurley JH. Mol Cell. 2012 Aug 10;47(3):339-48. doi: 10.1016/j.molcel.2012.05.027. Epub 2012 Jun 14. 10.1016/j.molcel.2012.05.027 PubMed 22704557
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