pF
(Plasmid #42519)

• Purpose: (Empty Backbone)
• Depositing Lab: Terry Magnuson
• Publication: Jang et al PLoS One. 2013;8(2):e57075. doi: 10.1371/journal.pone.0057075. Epub 2013 Feb 20.

Backbone

• Vector backbone: pBluescript KS-
• Backbone manufacturer: Stratagene
• Modifications to backbone: AmpR is replaced by wild-type FabI gene.
• Vector type: Bacterial Expression, Unspecified ; Cloning
• Promoter: T7, T3
• Selectable markers: FabI

Growth in Bacteria

• Bacterial Resistance(s): Triclosan
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Growth instructions: Can be propagated in any E. coli strain. When grown on agar plates, 32 deg C incubation will yield larger colonies compared to 37 deg C grown ones. The colony size difference varies depending on the host strain. In broth culture, 37 deg C incubation may produce higher yield of plasmid DNA. Use 1 micromolar triclosan for selection on plate and in broth cultures. Triclosan can be purchased from VWR (Cat. No. 80511-110. TRICLOSAN ANTIBIOTIC 1GM, Supplier: EMD Millipore, 647950-1GM). The triclosan powder is dissolved in pure DMSO in 50 millimolar and 1 millimolar (1000X) stocks and aliquoted, stored in -20.
• Copy number: High Copy

Cloning Information

• Cloning method: Ligation Independent Cloning

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry