pCDNA3-LOV-VP16
(Plasmid #42499)

• Purpose: LOV (N terminal domain of FKF1) fused to VP16, part of a light-inducible transcription factor
• Depositing Lab: Ricardo Dolmetsch
• Publication: Yazawa et al Nat Biotechnol. 2009 Oct;27(10):941-5. doi: 10.1038/nbt.1569. Epub 2009 Oct 4.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogene
• Backbone size w/o insert (bp): 5300
• Total vector size (bp): 6005
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FKF1 (N-LOV, G128D mutant) fused with VP16
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 678
• Mutation: G128D 1-168aa
• Promoter: CMV
• Tags / Fusion Proteins:
  • F-type VP16 activation domain (C terminal on insert)
  • HA tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: Not I (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry