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Addgene

KaiB
(Plasmid #42496)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42496 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pet47 b+
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5200
  • Total vector size (bp) 5476
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Growth instructions for expression: Grow at 37 C until OD600 reaches 0.6. Then chill flask on ice for 10 minutes. Add ITPG, and place back into incubator at 30C for three hours.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Kai B
  • Species
    cyanobacteria
  • Insert Size (bp)
    3200
  • GenBank ID
    CP000100
  • Promoter t7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer ccaggatcCGATGAGCCCTCGTAAAACCTACATTC
  • 3′ sequencing primer CAAGATTCCGACGACTTCTAATAGAAGCTtgcgg
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Plasmid originally cloned in Rust et al., Science, 318, 809-812 (2007).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    KaiB was a gift from Michael Rust (Addgene plasmid # 42496 ; http://n2t.net/addgene:42496 ; RRID:Addgene_42496)

  • For your References section:

    Robust and tunable circadian rhythms from differentially sensitive catalytic domains. Phong C, Markson JS, Wilhoite CM, Rust MJ. Proc Natl Acad Sci U S A. 2013 Jan 15;110(3):1124-9. doi: 10.1073/pnas.1212113110. Epub 2012 Dec 31. 10.1073/pnas.1212113110 PubMed 23277568
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