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Addgene

NISCHA
(Plasmid #42477)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42477 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pNIC28-Bsa4
  • Backbone manufacturer
    SGC
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Mach1 ;
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NISCHA
  • Alt name
    NISCH
  • Alt name
    gi|34783223
  • Alt name
    PDB:3P0C
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    421
  • Mutation
    See Comments
  • Entrez Gene
    NISCH (a.k.a. I-1, IR1, IRAS, hIRAS)
  • Tag / Fusion Protein
    • His6-TEV (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

http://www.thesgc.org/structures/3P0C/ Use BL21(DE3)-R3-pRARE2 strain for expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NISCHA was a gift from Nicola Burgess-Brown (Addgene plasmid # 42477 ; http://n2t.net/addgene:42477 ; RRID:Addgene_42477)