NISCHA
(Plasmid #42477)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Nicola Burgess-Brown
• Publication: SGC Oxford (unpublished)

Backbone

• Vector backbone: pNIC28-Bsa4
• Backbone manufacturer: SGC
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Mach1 ;
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NISCHA
• Alt name: NISCH
• Alt name: gi|34783223
• Alt name: PDB:3P0C
• Species: H. sapiens (human)
• Insert Size (bp): 421
• Mutation: See Comments
• Entrez Gene: NISCH (a.k.a. I-1, IR1, IRAS, hIRAS)
• Tag / Fusion Protein:
  • His6-TEV (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

http://www.thesgc.org/structures/3P0C/ Use BL21(DE3)-R3-pRARE2 strain for expression.