ITPAA
(Plasmid
#42345)
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PurposeBacterial expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 42345 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28-LIC
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Backbone manufacturerSGC
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Mach1 ;
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameITPAA
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Alt nameITPKB
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Alt namegi|15626999
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Alt namePDB:2CAR/2J4E
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SpeciesH. sapiens (human)
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Insert Size (bp)654
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MutationSee Comments
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GenBank IDITPKB gi|15626999
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Entrez GeneITPA (a.k.a. C20orf37, DEE35, HLC14-06-P, ITPase, My049, NTPase, dJ794I6.3)
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Tag
/ Fusion Protein
- His6-Thrombin (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
http://www.thesgc.org/structures/2CAR/ Use BL21(DE3)-R3-pRARE2 strain for expression.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ITPAA was a gift from Nicola Burgess-Brown (Addgene plasmid # 42345 ; http://n2t.net/addgene:42345 ; RRID:Addgene_42345)