pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro Notes
Addgene diagnostic digest
This gel depicts the NEB 1kb ladder and a digestion of the plasmid with the following enzyme(s): NotI and SpeI (separate).
We expected the following band sizes for this digest: both should linearize for 10.1kb bands. We see the expected pattern.
